Neutralizing Antibodies Targeting HIV-1 gp41
نویسندگان
چکیده
منابع مشابه
HIV-1 gp41 Core with Exposed Membrane-Proximal External Region Inducing Broad HIV-1 Neutralizing Antibodies
The membrane-proximal external region (MPER) of the HIV-1 gp41 consists of epitopes for the broadly cross-neutralizing monoclonal antibodies 2F5 and 4E10. However, antigens containing the linear sequence of these epitopes are unable to elicit potent and broad neutralizing antibody responses in vaccinated hosts, possibly because of inappropriate conformation of these epitopes. Here we designed a...
متن کاملHIV-1 Envelope gp41 Broadly Neutralizing Antibodies: Hurdles for Vaccine Development
1 Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, United States of America, 2 Department of Medicine, Duke University School of Medicine, Durham, North Carolina, United States of America, 3 Department of Pathology, Duke University School of Medicine, Durham, North Carolina, United States of America, 4 Department of Immunology, Duke University School of ...
متن کاملA fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies.
Most antibodies induced by HIV-1 are ineffective at preventing initiation or spread of infection because they are either nonneutralizing or narrowly isolate-specific. Rare, "broadly neutralizing" antibodies have been detected that recognize relatively conserved regions on the envelope glycoprotein. Using stringently characterized, homogeneous preparations of trimeric HIV-1 envelope protein in r...
متن کاملEliciting broadly neutralizing antibodies against HIV-1 that target gp41 MPER
Methods We generated a mini-protein that is structurally rigid, yet efficiently recognized by 2F5, 4E10 and Z13e1. It contains the C-terminal 54 a.a. of gp41 ectodomain (gp41-54Q), which includes the HR2 and the MPER. A 6xHis tag at the C-terminus was used to attach gp41-54Q to Zn-chitosan, which served as an antigen carrier/adjuvant. Rabbits were immunized subcutaneously, 4 times, using two di...
متن کاملEnvelope Deglycosylation Enhances Antigenicity of HIV-1 gp41 Epitopes for Both Broad Neutralizing Antibodies and Their Unmutated Ancestor Antibodies
The HIV-1 gp41 envelope (Env) membrane proximal external region (MPER) is an important vaccine target that in rare subjects can elicit neutralizing antibodies. One mechanism proposed for rarity of MPER neutralizing antibody generation is lack of reverted unmutated ancestor (putative naive B cell receptor) antibody reactivity with HIV-1 envelope. We have studied the effect of partial deglycosyla...
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ژورنال
عنوان ژورنال: Viruses
سال: 2020
ISSN: 1999-4915
DOI: 10.3390/v12111210